In vitro propagation of Jankea heldreichii Boiss. (Gesneriaceae)

Shoot cultures of Jankea heldreichii were established from seeds aspetically germinated on hormone-free MS medium. Various explants including cotyledone and hypocotyle fragments, axillary buds and whole shoots were transferred to MS medium supplemented with 5.0 mg/l BA and 0.1mg/l IBA for shoots induction. Shoots regenerated directly without intervening callus on types of explants. Most responsive were cotyledone fragments and whole shoots manifesting 80% and 100% shoot regeneration respectively. Shoot cultures were maintained on medium with BA decreased to 0.2-o.5 mg/l BA and rooted on medium with 0.5mg/l IBA.